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CD24 Recombinant antibody

CD24 Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241691B2

Cat no : 84342-5-RR

Print datasheet

Synonyms

nectadrin, Ly 52, HSA, CD24a antigen, Cd24a

验证数据展示

  • IF Staining of Neuro-2a using 84342-5-RR

    IF Staining of Neuro-2a using 84342-5-RR

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using CD24 antibody (84342-5-RR, Clone: 241691B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84342-5-RR

    Affinity and Kinetic Characterization of 84342-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84342-5-RR against Mouse CD24 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive IF/ICC detected inNeuro-2a cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84342-5-RR targets CD24 in IF/ICC, ELISA applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD24a antigen
Synonyms nectadrin, Ly 52, HSA, CD24a antigen, Cd24a
Calculated Molecular Weight8kDa
GenBank Accession NumberNM_009846.2
Gene Symbol CD24
Gene ID (NCBI) 12484
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24807
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD24 (known as heat stable antigen) is a small highly glycosylated GPI-linked sialoprotein. It is normally expressed at the surface of most B lymphocytes and differentiating neuroblasts, and it is also up-regulated in a wide variety of cancers. Studies have shown that CD24 functions in the regulation of B-cell apoptosis, leukocyte signal transduction, and leukocyte adhesion. Since it is highly glycosylated, the apparent molecular weight of CD24 could be variable, ranging from 30 kDa to 70 kDa. (Ref: Akihiko Sano, MD., 2009)

实验方案

Product Specific Protocols
IF protocol for CD24 antibody 84342-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols