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CD244 Recombinant antibody

CD244 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241260C6

Cat no : 84086-1-RR

Print datasheet

Synonyms

SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4

验证数据展示

  • WB analysis using 84086-1-RR

    WB analysis using 84086-1-RR

    NK-92 cells were subjected to SDS PAGE followed by western blot with 84086-1-RR (CD244 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84086-1-RR

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84086-1-RR

    IHC staining of human tonsillitis using 84086-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84086-1-RR (CD244 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84086-1-RR

    Affinity and Kinetic Characterization of 84086-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84086-1-RR against Human CD244 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inNK-92 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84086-1-RR is CD244 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD244 molecule, natural killer cell receptor 2B4
Synonyms SLAMF4, NKR2B4, NK cell activation-inducing ligand, NAIL, h2B4
Calculated Molecular Weight 365 aa, 41 kDa
GenBank Accession NumberBC053985
Gene Symbol CD244
Gene ID (NCBI) 51744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9BZW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD244, also known as SLAMF4 or 2B4, is a type I transmembrane glycoprotein belonging to the signaling lymphocyte activation molecule (SLAM) family. It consists of an extracellular segment with two immunoglobulin (Ig)-like domains, a transmembrane region, and a cytoplasmic domain containing tyrosine-based motifs (PMID: 9841922; 30546369). CD244 is present on natural killer (NK) cells, γδ T cells, a subset of CD8+ T cells, monocytes, basophils, dendritic cells, and myeloid-derived suppressor cells (MDSCs) (PMID: 30546369). It binds to CD48 with high affinity and transmits stimulatory or inhibitory signals that regulate immune function (PMID: 9841922; 18523281).

实验方案

Product Specific Protocols
WB protocol for CD244 antibody 84086-1-RRDownload protocol
IHC protocol for CD244 antibody 84086-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols