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CoraLite® Plus 750 Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody

CD26 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

BA5b

Cat no : CL750-65574

Print datasheet

Synonyms

dipeptidyl peptidase 4, BA5b, ADCP-2, ADCP2, ADCP 2

验证数据展示

  • FC experiment of human PBMCs using CL750-65574

    FC experiment of human PBMCs using CL750-65574

    1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 5 ul CoraLite® Plus 750 Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody (CL750-65574, Clone: BA5b) (right), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (left). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using CL750-65574

    FC experiment of human PBMCs using CL750-65574

    1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD26 (BA5b) Mouse IgG2a Recombinant Antibody (CL750-65574, Clone: BA5b) (red), or CoraLite® Plus 750 Mouse IgG2a Isotype Control (C1.18.4) (CL750-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs
For other applications, we recommend the unconjugated version of this antibody, 65574-1-MR

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-65574 targets CD26 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen A human T cell clone 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name dipeptidyl-peptidase 4
Synonyms dipeptidyl peptidase 4, BA5b, ADCP-2, ADCP2, ADCP 2
Calculated Molecular Weight 88 kDa
GenBank Accession NumberBC013329
Gene Symbol DPP4
Gene ID (NCBI) 1803
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD26, also known as DPP4 (dipeptidyl peptidase-4), is a 110 kDa type II cell-surface glycoprotein widely expressed on T cells, activated B cells, activated NK cells and myeloid cells as well as on epithelial cells, fibroblasts, mesothelium, and endothelial cells of a variety of tissues (PMID:33777580; 26919392). It has ecto-enzyme activity in its extracellular domain and cleaves amino-terminal dipeptides with either L-proline or L-alanine at the penultimate position (PMID: 9553764). CD26 plays roles in diverse biological processes such as immunoregulation, glucose homeostasis, and tumorigenesis (PMID: 33777580).

实验方案

Product Specific Protocols
FC protocol for CL Plus 750 CD26 antibody CL750-65574Download protocol
Standard Protocols
Click here to view our Standard Protocols