• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

FITC Plus Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody

CD27 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

FITC Plus Fluorescent Dye

CloneNo.

O323

Cat no : FITC-65627

Print datasheet

Synonyms

T cell activation antigen CD27, S152, O323, CD27L receptor, CD27 molecule

验证数据展示

  • FC experiment of human PBMCs using FITC-65627

    FC experiment of human PBMCs using FITC-65627

    1x10^6 human PBMCs were surface stained with APC Plus Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3) and 5 ul FITC Plus Anti-Human CD27 (O323) Mouse IgG2a Recombinant Antibody (FITC-65627, Clone:O323) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were not fixed.L ymphocytes were gated.

  • FC experiment of human PBMCs using FITC-65627

    FC experiment of human PBMCs using FITC-65627

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD27(O323) Mouse IgG2a Recombinant Antibody (FITC-65627, Clone: O323) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65627 targets CD27 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD27 molecule
Synonyms T cell activation antigen CD27, S152, O323, CD27L receptor, CD27 molecule
Calculated Molecular Weight 29 kDa
GenBank Accession NumberBC012160
Gene Symbol CD27
Gene ID (NCBI) 939
ENSEMBL Gene IDENSG00000139193
Conjugate FITC Plus Fluorescent Dye
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD27 (also known as TNFRSF7) is a type I glycoprotein expressed on some B cells and the majority of T cells, and is a member of the tumor necrosis factor (TNF) receptor family. CD27 is required for generation and long-term maintenance of T cell immunity (PMID: 11062504). It is a receptor for CD70 (CD27L). Ligation of CD27 by CD70 induces strong ubiquitination of TRAF and the activation of both canonical and non-canonical NF-kappaB pathways, as well as the JNK pathway (PMID: 19426224). CD27 may also play a role in apoptosis through association with SIVA1.

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD27 antibody FITC-65627Download protocol
Standard Protocols
Click here to view our Standard Protocols