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PE Anti-Mouse ICOSLG/B7-H2/CD275 (HK5.3)

ICOSLG/B7-H2/CD275 Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse

Applications

FC

Conjugate

PE Fluorescent Dye

CloneNo.

HK5.3

Cat no : PE-65114

Print datasheet

Synonyms

CD275, B7-H2, Icosl, icos ligand, ICOS L

验证数据展示

  • FC experiment of mouse splenocytes using PE-65114

    FC experiment of mouse splenocytes using PE-65114

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE Anti-Mouse CD275 (B7-H2) (PE-65114, Clone: HK5.3). Cells were not fixed.

  • FC experiment of mouse splenocytes using PE-65114

    FC experiment of mouse splenocytes using PE-65114

    1X10^6 C57BL/6 mouse splenocytes were surface stained with APC Anti-Mouse CD3 (APC-65077, Clone: 17A2) and 0.06 ug PE-Rat IgG2a isotype control. Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

PE-65114 targets ICOSLG/B7-H2/CD275 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC Application Description
Tested Reactivity Mouse
Immunogen Mouse B7-RP1 transfected cell line 种属同源性预测
Host / Isotype Rat / IgG2a, kappa
Class Monoclonal
Type Antibody
Full Name icos ligand
Synonyms CD275, B7-H2, Icosl, icos ligand, ICOS L
GenBank Accession NumberBC029227
Gene Symbol CD275
Gene ID (NCBI) 50723
RRIDAB_2883903
Conjugate PE Fluorescent Dye
Excitation/Emission Maxima Wavelengths496 nm, 565 nm / 578 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDQ9JHJ8
Storage Buffer Phosphate based buffer with 0.09% sodium azide and 0.1% gelatin, pH 7.2.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD275, also known as B7-H2, ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. CD275 is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of CD275/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).

实验方案

Product Specific Protocols
FC protocol for PE ICOSLG/B7-H2/CD275 antibody PE-65114Download protocol
Standard Protocols
Click here to view our Standard Protocols