验证数据展示
经过测试的应用
| Positive FC detected in | mouse splenocytes |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
PE-65114 targets ICOSLG/B7-H2/CD275 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Mouse B7-RP1 transfected cell line 种属同源性预测 |
| 宿主/亚型 | Rat / IgG2a, kappa |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | icos ligand |
| 别名 | CD275, B7-H2, Icosl, icos ligand, ICOS L |
| GenBank蛋白编号 | BC029227 |
| 基因名称 | CD275 |
| Gene ID (NCBI) | 50723 |
| RRID | AB_2883903 |
| 偶联类型 | PE Fluorescent Dye |
| 最大激发/发射波长 | 496 nm, 565 nm / 578 nm |
| 形式 | Liquid |
| 纯化方式 | Affinity purification |
| UNIPROT ID | Q9JHJ8 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.1% gelatin , pH 7.2 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD275, also known as B7-H2, ICOSLG or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. CD275 is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of CD275/ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for PE ICOSLG/B7-H2/CD275 antibody PE-65114 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |