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- KD/KO Validated
B7-H3/CD276 Polyclonal antibody
B7-H3/CD276 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, LNCaP cells, U2OS cells |
| Positive IHC detected in | human prostate cancer tissue, human testis tissue, human medulloblastoma tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14453-1-AP targets B7-H3/CD276 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | B7-H3/CD276 fusion protein Ag6006 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD276 molecule |
| Synonyms | 4Ig B7 H3, B7 homolog 3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352 |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC062581 |
| Gene Symbol | CD276 |
| Gene ID (NCBI) | 80381 |
| RRID | AB_2073577 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5ZPR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for B7-H3/CD276 antibody 14453-1-AP | Download protocol |
| IHC protocol for B7-H3/CD276 antibody 14453-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Nutr Biochem Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression | ||
J Cell Physiol Downregulated miR-187 contributes to the keratinocytes hyperproliferation in psoriasis. | ||
CNS Neurosci Ther PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma. | ||
J Cancer The immunoregulatory protein B7-H3 promotes aerobic glycolysis in oral squamous carcinoma via PI3K/Akt/mTOR pathway. | ||
Med Oncol Overexpression of B7-H3 in CD14+ monocytes is associated with renal cell carcinoma progression. | ||
J Oncol Alpha-Fetoprotein Regulates the Expression of Immune-Related Proteins through the NF-κB (P65) Pathway in Hepatocellular Carcinoma Cells. |
