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CD28 Polyclonal antibody

CD28 Polyclonal Antibody for IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26174-1-AP

Print datasheet

Synonyms

CD28, CD28 molecule, Tp44

验证数据展示

  • IP experiment of Jurkat using 26174-1-AP

    IP experiment of Jurkat using 26174-1-AP

    IP result of anti-CD28 (IP:26174-1-AP, 4ug; Detection:26174-1-AP 1:400) with Jurkat cells lysate 1400 ug.

  • IHC staining of human tonsillitis using 26174-1-AP

    IHC staining of human tonsillitis using 26174-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26174-1-AP (CD28 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 26174-1-AP

    IHC staining of human tonsillitis using 26174-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 26174-1-AP (CD28 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IP detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26174-1-AP targets CD28 in IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IP, IHC, ELISA Application Description
Tested Reactivity human
Immunogen CD28 fusion protein Ag24058 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD28 molecule
Synonyms CD28, CD28 molecule, Tp44
Calculated Molecular Weight 220 aa, 25 kDa
Observed Molecular Weight44 kDa
GenBank Accession NumberBC093698
Gene Symbol CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP10747
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

实验方案

Product Specific Protocols
IHC protocol for CD28 antibody 26174-1-APDownload protocol
IP protocol for CD28 antibody 26174-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols