Anti-Human CD28 (CD28.2)

CD28 Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

FC and More (1)

Conjugate

Unconjugated

CloneNo.

CD28.2

Cat No : 65099-1-Ig

Print datasheet

Synonyms

CD28, CD28 molecule, Tp44



经过测试的应用

Positive FC detected inhuman peripheral blood lymphocytes
Planning an IF experiment? We recommend our CoraLite®647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

发表文章中的应用

IHCSee 1 publications below

产品信息

65099-1-Ig targets CD28 in IHC, FC applications and shows reactivity with Human samples.

Tested Applications FC
Cited ApplicationsIHC
Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen N/A
Full Name CD28 molecule
Synonyms CD28, CD28 molecule, Tp44
Calculated molecular weight 220 aa, 25 kDa
GenBank accession numberBC093698
Gene symbol CD28
UniprotP10747
Gene ID (NCBI) 940
RRIDAB_2918401
Conjugate Unconjugated
Form Liquid
Purification Method Affinity purification
Storage Buffer Phosphate based buffer with 0.09% sodium azide, pH 7.2.
Storage ConditionsStore at 2-8°C.

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).

实验方案

Product Specific Protocols
FC protocol for CD28 antibody 65099-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancer Cell Int

Comprehensive analysis of prognostic immune-related genes and drug sensitivity in cervical cancer.

Authors - Ya-Nan Pi