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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody (65621-1-MR, Clone: CD28.2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65621-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody (65621-1-MR, Clone: CD28.2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65621-1-PBS in a different storage buffer formulation.

Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody, PBS Only

CD28 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65621-1-PBS

产品说明书

CloneNo. CD28.2

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

FC

别名

CD28 molecule, CD28.2, T-cell-specific surface glycoprotein CD28, Tp44

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

65621-1-PBS targets CD28 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 N/A 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Recombinant
产品类型 Antibody
全称 CD28 molecule
别名 CD28 molecule, CD28.2, T-cell-specific surface glycoprotein CD28, Tp44
计算分子量 220 aa, 25 kDa
GenBank蛋白编号BC093698
基因名称 CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).