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Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody, PBS Only

CD28 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

CD28.2

Cat no : 65621-1-PBS

Print datasheet

Synonyms

Tp44, T-cell-specific surface glycoprotein CD28, CD28.2, CD28 molecule

验证数据展示

  • FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody (65621-1-MR, Clone: CD28.2) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65621-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    FC experiment of human PBMCs using 65621-1-MR (same clone as 65621-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD28 (CD28.2) Mouse IgG2a Recombinant Antibody (65621-1-MR, Clone: CD28.2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue), and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed. This data was developed using the same antibody clone with 65621-1-PBS in a different storage buffer formulation.

产品信息

65621-1-PBS targets CD28 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD28 molecule
Synonyms Tp44, T-cell-specific surface glycoprotein CD28, CD28.2, CD28 molecule
Calculated Molecular Weight 220 aa, 25 kDa
GenBank Accession NumberBC093698
Gene Symbol CD28
Gene ID (NCBI) 940
ENSEMBL Gene IDENSG00000178562
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD28 (T-cell-specific surface glycoprotein CD28), also known as T44 and Tp44, is a 44 kD disulfide-linked homodimeric type I glycoprotein (PMID: 2162180). It is a member of the immunoglobulin superfamily and is expressed on most T lineage cells, NK cell subsets, and plasma cells (PMID: 2162180, 8386518). CD28 may affect in vivo immune responses by functioning both as a cell adhesion molecule linking B and T lymphocytes and as the surface component of a novel signal transduction pathway (PMID: 2162180, 3021470). CD28 binds both CD80 and CD86 with a highly conserved motif MYPPY in the CDR3-like loop (PMID: 15696168, 7964482). CD28 is considered a major co-stimulatory molecule, inducing T lymphocyte activation and IL-2 synthesis, and preventing cell death (PMID: 1348520).