CD28 Recombinant antibody, PBS Only

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF, ELISA

Conjugate

Unconjugated

CloneNo.

240354D2

Cat No : 83383-6-PBS

Synonyms

CD28, CD28 molecule, Tp44

IF Staining of Jurkat using 83383-6-RR (same clone as 83383-6-PBS)
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells and THP-1 cells (negative control) using CD28 antibody (83383-6-RR, Clone: 240354D2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83383-6-PBS in a different storage buffer formulation.
Affinity experiments of 83383-6-RR
Biolayer interferometry (BLl) kinetic assays of 83383-6-RR against Human CD28 were performed. The affinity constant is 1.99 nM.

Size

The immunogen of 83383-6-PBS is CD28 Fusion Protein expressed in E. coli.

Tested Applications	IF, ELISA Application Description
Tested Reactivity	Human
Immunogen	Fusion Protein 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Full Name	CD28 molecule
Synonyms	CD28, CD28 molecule, Tp44
Calculated Molecular Weight	220 aa, 25 kDa
GenBank Accession Number	BC093698
Gene Symbol	CD28
Gene ID (NCBI)	940
ENSEMBL Gene ID	ENSG00000178562
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purfication
UNIPROT ID	P10747
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C.