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验证数据展示

  • WB analysis using 32089-1-AP

    WB analysis using 32089-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 32089-1-AP (CD2AP antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 32089-1-AP

    IF Staining of HepG2 using 32089-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cd2ap antibody (32089-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CD2AP Polyclonal antibody

CD2AP Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 32089-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

CMS, CD2-associated protein, CD2 Associated Protein, Cas ligand with multiple SH3 domains, Adapter protein CMS

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, mouse testis tissue, Daudi cells, Jurkat cells, K-562 cells, rat testis tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32089-1-AP targets CD2AP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CD2AP fusion protein Ag36919 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD2-associated protein
别名 CMS, CD2-associated protein, CD2 Associated Protein, Cas ligand with multiple SH3 domains, Adapter protein CMS
计算分子量71 kDa，639aa
观测分子量71 kDa
GenBank蛋白编号NM_012120.2
基因名称 CD2AP
Gene ID (NCBI) 23607
RRIDAB_3670188
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y5K6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD2 associated protein (CD2AP, also known as CMS) is a scaffolding molecule that acts as an adapter protein between membrane proteins and the actin cytoskeleton (PMID: 10339567). CD2AP also plays a role in epithelial cell junction formation and affects the pathogenesis of Alzheimer's disease (PMID: 22891260; 31440393). Its mutations are associated with sporadic nephrotic syndrome and focal segmental glomerulosclerosis (FSGS) (PMID: 17713465; 19131354).

实验方案

Product Specific Protocols
WB protocol for CD2AP antibody 32089-1-APDownload protocol
IF protocol for CD2AP antibody 32089-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols