FITC Anti-Mouse CD3ε (500-A2)
CD3ε Monoclonal Antibody for FC
Host / Isotype
Hamster / IgG
Reactivity
Mouse
Applications
FC
Conjugate
FITC Fluorescent Dye
CloneNo.
500-A2
验证数据展示
经过测试的应用
Positive FC detected in | mouse splenocytes |
推荐稀释比
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
FITC-65061 targets CD3ε in FC applications and shows reactivity with Mouse samples.
Tested Applications | FC Application Description |
Tested Reactivity | Mouse |
Immunogen | Immune complexes isolated from C6VL-BS thymoma cells 种属同源性预测 |
Host / Isotype | Hamster / IgG |
Class | Monoclonal |
Type | Antibody |
Full Name | CD3 antigen, epsilon polypeptide |
Synonyms | AI504783, CD3, CD3e, CD3epsilon, CD3ε, T3e |
GenBank Accession Number | BC098236 |
Gene Symbol | CD3ε |
Gene ID (NCBI) | 12501 |
RRID | AB_2883751 |
Conjugate | FITC Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 498 nm / 526 nm |
Form | Liquid |
Purification Method | Affinity purification |
UNIPROT ID | P22646 |
Storage Buffer | PBS with 0.1% sodium azide. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).
实验方案
Product Specific Protocols | |
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FC protocol for FITC CD3ε antibody FITC-65061 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |