• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody, PBS Only

CD3 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

UCHT1

Cat no : 65570-1-PBS

Print datasheet

Synonyms

CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E

验证数据展示

  • FC experiment of human PBMCs using 65570-1-MR (same clone as 65570-1-PBS)

    FC experiment of human PBMCs using 65570-1-MR (same clone as 65570-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 Mouse IgG2a Recombinant Antibody (65570-1-MR, Clone: UCHT1) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65570-1-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 65570-1-MR (same clone as 65570-1-PBS)

    FC experiment of human PBMCs using 65570-1-MR (same clone as 65570-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD3 (UCHT1) Mouse IgG2a Recombinant Antibody (65570-1-MR, Clone: UCHT1) or 0.25 ug 0.25 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4), and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 65570-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

产品信息

65570-1-PBS targets CD3 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3E, CD3 epsilon, T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, T3E
Calculated Molecular Weight 207 aa, 23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C. Stable for one year after shipment.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a multimeric protein associated with the T-cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction (PMID: 15885124). CD3 is composed of CD3γ, δ, ε, and ζ chains (PMID: 1826255). It is expressed by thymocytes in a developmentally regulated manner, T cells, and some NK cells (PMID: 3289580). The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction (PMID: 11985657).