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CD3 Recombinant antibody, PBS Only

CD3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242012G2

Cat no : 84505-4-PBS

Print datasheet

Synonyms

CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon

验证数据展示

  • WB analysis using 84505-4-RR (same clone as 84505-4-PBS)

    WB analysis using 84505-4-RR (same clone as 84505-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84505-4-RR (CD3E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 84505-4-RR (same clone as 84505-4-PBS)

    IF Staining of Jurkat using 84505-4-RR (same clone as 84505-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD3 antibody (84505-4-RR, Clone: 242012G2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84505-4-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD3 Ready-To-Use IHC Kit. CD3 primary antibody included.

产品信息

84505-4-PBS targets CD3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD3e molecule, epsilon (CD3-TCR complex)
Synonyms CD3E, T-cell surface antigen T3/Leu-4 epsilon chain, T3E, CD3-epsilon, CD3 epsilon
Calculated Molecular Weight 207 aa, 23 kDa
Observed Molecular Weight23 kDa
GenBank Accession NumberBC049847
Gene Symbol CD3
Gene ID (NCBI) 916
ENSEMBL Gene IDENSG00000198851
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP07766
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This antibody is directed against the epsilon chain of human CD3 molecule.