• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 13334-1-AP

    WB analysis using 13334-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13334-1-AP

    IP experiment of K-562 using 13334-1-AP

    IP result of anti-CD300LF (IP:13334-1-AP, 4ug; Detection:13334-1-AP 1:1500) with K-562 cells lysate 4000ug.

CD300LF Polyclonal antibody

CD300LF Polyclonal Antibody for WB, IP, ELISA
Cat No. 13334-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IP, ELISA

别名

CMRF35 like molecule 1, CLM-1, CLM1, CLM 1, CD300f

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inK-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13334-1-AP targets CD300LF in WB, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 CD300LF fusion protein Ag4150 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD300 molecule-like family member f
别名 CMRF35 like molecule 1, CLM-1, CLM1, CLM 1, CD300f
计算分子量 290 aa, 32 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC028199
基因名称 CD300LF
Gene ID (NCBI) 146722
RRIDAB_2877941
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TDQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.

实验方案

Product Specific Protocols
WB protocol for CD300LF antibody 13334-1-APDownload protocol
IP protocol for CD300LF antibody 13334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

BMC Pulm Med

Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study.

Authors - Dmitry N Grigoryev
View Article
humanWB

J Inflamm Res

Quercetin Attenuates MRGPRX2-Mediated Mast Cell Degranulation via the MyD88/IKK/NF-κB and PI3K/AKT/ Rac1/Cdc42 Pathway

Authors - Chenrui Zhao
View Article