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CD300LF Polyclonal antibody

CD300LF Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 13334-1-AP

Print datasheet

Synonyms

CMRF35 like molecule 1, CLM-1, CLM1, CLM 1, CD300f

验证数据展示

  • WB analysis using 13334-1-AP

    WB analysis using 13334-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13334-1-AP (CD300LF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 13334-1-AP

    IP experiment of K-562 using 13334-1-AP

    IP result of anti-CD300LF (IP:13334-1-AP, 4ug; Detection:13334-1-AP 1:1500) with K-562 cells lysate 4000ug.

经过测试的应用

Positive WB detected inJurkat cells, U-937 cells
Positive IP detected inK-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

13334-1-AP targets CD300LF in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen CD300LF fusion protein Ag4150 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD300 molecule-like family member f
Synonyms CMRF35 like molecule 1, CLM-1, CLM1, CLM 1, CD300f
Calculated Molecular Weight 290 aa, 32 kDa
Observed Molecular Weight 30-34 kDa
GenBank Accession NumberBC028199
Gene Symbol CD300LF
Gene ID (NCBI) 146722
RRIDAB_2877941
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDQ1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD300LF, also known as CLM1 (CMRF35-like molecule 1), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LF is an inhibitory receptor that has been shown to inhibit various inflammatory processes in myeloid cells, such as macrophages and mast cells.

实验方案

Product Specific Protocols
WB protocol for CD300LF antibody 13334-1-APDownload protocol
IP protocol for CD300LF antibody 13334-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

BMC Pulm Med

Identification of new biomarkers for Acute Respiratory Distress Syndrome by expression-based genome-wide association study.

Authors - Dmitry N Grigoryev
View Article
humanWB

J Inflamm Res

Quercetin Attenuates MRGPRX2-Mediated Mast Cell Degranulation via the MyD88/IKK/NF-κB and PI3K/AKT/ Rac1/Cdc42 Pathway

Authors - Chenrui Zhao
View Article