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验证数据展示

  • WB analysis of human kidney using 15426-1-AP

    WB analysis of human kidney using 15426-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 15426-1-AP (CD300LG antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 15426-1-AP

    IF Staining of HEK-293 using 15426-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 15426-1-AP (CD300LG antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CD300LG Polyclonal antibody

CD300LG Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 15426-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CMRF35 like molecule 9, CLM-9, CLM9, CLM 9, CD300g

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman kidney tissue, HepG2 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15426-1-AP targets CD300LG in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD300LG fusion protein Ag8075 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD300 molecule-like family member g
别名 CMRF35 like molecule 9, CLM-9, CLM9, CLM 9, CD300g
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC025395
基因名称 CD300LG
Gene ID (NCBI) 146894
RRIDAB_2074217
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6UXG3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD300LG, also known as CLM9 (CMRF35-like molecule 9), is a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. CD300LG is a receptor which may mediate L-selectin-dependent lymphocyte rollings and may play an important role in inflammation.

实验方案

Product Specific Protocols
WB protocol for CD300LG antibody 15426-1-APDownload protocol
IF protocol for CD300LG antibody 15426-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols