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Anti-Human CD31 (WM-59)

CD31 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

human

Applications

IF/ICC, FC

Conjugate

Unconjugated

CloneNo.

WM-59

Cat no : 65268-1-Ig

Print datasheet

Synonyms

PECAM1, PECAM 1, PECA1, GPIIA', GPIIA

验证数据展示

  • IF Staining of HUVEC using 65268-1-Ig

    IF Staining of HUVEC using 65268-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using CD31 antibody (65268-1-Ig, Clone: WM-59 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004).

  • FC experiment of human PBMCs using 65268-1-Ig

    FC experiment of human PBMCs using 65268-1-Ig

    1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD31 (65268-1-Ig, Clone:WM-59) and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 or unstained. Cells were not fixed. Monocytes were gated.

经过测试的应用

Positive IF/ICC detected inHUVEC cells
Positive FC detected inhuman PBMCs
Planning an IHC experiment? We recommend our IHCeasy CD31 Ready-To-Use IHC Kit. CD31 primary antibody included.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65268-1-Ig targets CD31 in IF/ICC, FC applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name platelet/endothelial cell adhesion molecule
Synonyms PECAM1, PECAM 1, PECA1, GPIIA', GPIIA
Calculated Molecular Weight 83 kDa
GenBank Accession NumberBC022512
Gene Symbol CD31
Gene ID (NCBI) 5175
ENSEMBL Gene IDENSG00000261371
Conjugate Unconjugated
Form Liquid
Purification MethodAffinity purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD31, also known as PECAM1 (Platelet endothelial cell adhesion molecule), is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. It is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, CD31 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

实验方案

Product Specific Protocols
IF protocol for CD31 antibody 65268-1-IgDownload protocol
FC protocol for CD31 antibody 65268-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols