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APC Anti-Human CD314/NKG2D (1D11)

CD314/NKG2D Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG1, kappa

Reactivity

Human

Applications

FC

Conjugate

APC Fluorescent Dye

CloneNo.

1D11

Cat no : APC-65188

Print datasheet

Synonyms

CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D

验证数据展示

  • FC experiment of human PBMCs using APC-65188

    FC experiment of human PBMCs using APC-65188

    1X10^6 human PBMCs were surface co-stained with CL488 Anti-Human CD8a (CL488-65204, Clone: UCHT4) and 10 ul APC Anti-Human CD314/NKG2D (APC-65188, Clone:1D11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using APC-65188

    FC experiment of human PBMCs using APC-65188

    1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD314/NKG2D (APC-65188, Clone:1D11) or Mouse IgG1 Isotype Control. Cells were not fixed. Lymphocytes were gated.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 μl per 10^6 cells in a 100 µl suspension or 10 μl per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

APC-65188 targets CD314/NKG2D in FC applications and shows reactivity with Human samples.

Tested Applications FC Application Description
Tested Reactivity Human
Immunogen NKL cell line 种属同源性预测
Host / Isotype Mouse / IgG1, kappa
Class Monoclonal
Type Antibody
Full Name killer cell lectin-like receptor subfamily K, member 1
Synonyms CD314, D12S2489E, FLJ17759, FLJ75772, KLR, KLRK1, NK cell receptor D, NKG2 D, NKG2 D activating NK receptor, NKG2D
Calculated Molecular Weight 25 kDa
GenBank Accession NumberBC039836
Gene Symbol KLRK1
Gene ID (NCBI) 22914
RRIDAB_2883048
Conjugate APC Fluorescent Dye
Excitation/Emission Maxima Wavelengths650 nm / 660 nm
Form Liquid
Purification MethodThe purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
UNIPROT IDP26718
Storage Buffer PBS with 0.1% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD314, also known as NKG2D or Killer cell lectin-like receptor subfamily K member 1 (KLRK1), is a type II lectin-like transmembrane stimulatory receptor (PMID: 8436421). In humans, it is expressed on NK cells, gamma delta T cells, and CD8+ alpha beta T cells (PMID: 10426993). Various families of cell surface ligands have been identified, including the MICA/MICB and ULBP proteins (PMID: 12150888). CD314 is involved in both innate and adaptive immunities, and the NKG2D/NKG2DL pathway involves multiple effector cell types for controlling tumor progression (PMID: 31720075).

实验方案

Product Specific Protocols
FC protocol for APC CD314/NKG2D antibody APC-65188Download protocol
Standard Protocols
Click here to view our Standard Protocols