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验证数据展示

  • WB analysis using 24145-1-AP

    WB analysis using 24145-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24145-1-AP (CD320 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 24145-1-AP

    IHC staining of human tonsillitis using 24145-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24145-1-AP (CD320 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Raji using 24145-1-AP

    IF Staining of Raji using 24145-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD320 antibody (24145-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD320 Polyclonal antibody

CD320 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24145-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Transcobalamin receptor, TCblR, FDC-SM-8D6, FDC-signaling molecule 8D6, 8D6A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells, HeLa cells, K-562 cells, Raji cells, human placenta tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24145-1-AP targets CD320 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CD320 fusion protein Ag21445 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD320 molecule
别名 Transcobalamin receptor, TCblR, FDC-SM-8D6, FDC-signaling molecule 8D6, 8D6A
计算分子量 29 kDa
观测分子量70 kDa
GenBank蛋白编号BC000668
基因名称 CD320
Gene ID (NCBI) 51293
RRIDAB_3669431
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NPF0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD320, also known as 8D6, 8D6A or TCblR, is a single-pass type I membrane protein containing 2 LDL-receptor class A domains. Expressed abundantly on follicular dendritic cells (FDCs), CD320 has been shown to enhance proliferation of germinal center (GC) B cells. It is the receptor for cellular uptake of transcobalamin-bound cobalamin. Defects in CD320 are the cause of methylmalonic aciduria type TCblR (MMATC). CD320 has a calculated molecular mass of 29 kDa.The apparent molecular weight detected by this antibody is 70 kDa. The aberrant mobility of the protein by SDS-PAGE is probably caused by extensive complex glycosylation (PMID: 18779389; 23415653).

实验方案

Product Specific Protocols
WB protocol for CD320 antibody 24145-1-APDownload protocol
IHC protocol for CD320 antibody 24145-1-APDownload protocol
IF protocol for CD320 antibody 24145-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols