CoraLite®594 Anti-Human CD324 (E-cadherin) Rabbit Recombinant Antibody
CD324 (E-cadherin) Uni-rAbTM Recombinant Antibody for FC
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
240159B4
验证数据展示
经过测试的应用
Positive FC detected in | HT-29 cells |
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
推荐稀释比
Application | Dilution |
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This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL594-98030-2 is CD324 (E-cadherin) Fusion Protein expressed in E. coli.
Tested Applications | FC Application Description |
Tested Reactivity | human |
Immunogen | CD324 (E-cadherin) fusion protein Ag14973 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | E-cadherin, CD324, CAM 120/80, Cadherin-1, Cadherin 1 |
Calculated Molecular Weight | 882 aa, 97 kDa |
GenBank Accession Number | BC141838 |
Gene Symbol | E-cadherin |
Gene ID (NCBI) | 999 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.09% sodium azide and 0.5% BSA. |
Storage Conditions | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CD324, CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
实验方案
Product Specific Protocols | |
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FC protocol for CL594 CD324 (E-cadherin) antibody CL594-98030-2 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |