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CD32A/C Polyclonal antibody

CD32A/C Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 25323-1-AP

Print datasheet

Synonyms

CD32, CD32C, FcgammaRIIC, FCGR2B, FCGR2C, FcRIIC, hFcRII C

验证数据展示

  • WB analysis using 25323-1-AP

    WB analysis using 25323-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25323-1-AP (CD32A/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, U-937 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25323-1-AP targets CD32A/C in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen CD32A/C fusion protein Ag18123 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Fc fragment of IgG, low affinity IIc, receptor for (CD32)
Synonyms CD32, CD32C, FcgammaRIIC, FCGR2B, FCGR2C, FcRIIC, hFcRII C
Calculated Molecular Weight 323 aa, 36 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC137397
Gene Symbol CD32A/C
Gene ID (NCBI) 9103
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31995
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgG Fc receptors (FcγRs) play important roles in immune responses. The low-affinity IgG Fc receptor, FcγRII (CD32), consists of a family of primarily cell membrane receptor proteins: CD32A, CD32B, and CD32C. They are encoded by the mRNA splice variants of three highly related genes: FCGR2A, FCGR2B, and FCGR2C (PMID: 30941127). CD32 is present on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. This antibody raised against 254-323 aa of human CD32C (FCGR2C) can recognize both CD32A and CD32C forms.

实验方案

Product Specific Protocols
WB protocol for CD32A/C antibody 25323-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols