CD33 Monoclonal antibody

CD33 Monoclonal Antibody for WB, IF, IHC, ELISA
Cat No. 67135-1-Ig

产品说明书

CloneNo. 1B2E5

宿主/亚型

Mouse / IgG2a

种属反应性

Human and More (1)

应用

WB, IF, IHC, ELISA

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67135-1-Ig targets CD33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IF, IHC, ELISA Application Description
文献引用应用IF
经测试反应性 Human
文献引用反应性human
免疫原 CD33 fusion protein Ag11402 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 CD33 molecule
别名 CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3
计算分子量 364 aa, 40 kDa
观测分子量 67-70 kDa
GenBank蛋白编号BC028152
基因名称 CD33
Gene ID (NCBI) 945
RRIDAB_2882434
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20138
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

实验方案

Product Specific Protocols
WB protocol for CD33 antibody 67135-1-IgDownload protocol
IHC protocol for CD33 antibody 67135-1-IgDownload protocol
IF protocol for CD33 antibody 67135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Med (Lausanne)

Prognostic significance of peripheral and tumor-infiltrating lymphocytes in newly diagnosed stage III/IV non-small-cell lung cancer

Authors - Fenge Li
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