验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, HL-60 cells |
Positive IHC detected in | human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
67135-1-Ig targets CD33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF, IHC, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | CD33 fusion protein Ag11402 种属同源性预测 |
宿主/亚型 | Mouse / IgG2a |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | CD33 molecule |
别名 | CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3 |
计算分子量 | 364 aa, 40 kDa |
观测分子量 | 67-70 kDa |
GenBank蛋白编号 | BC028152 |
基因名称 | CD33 |
Gene ID (NCBI) | 945 |
RRID | AB_2882434 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P20138 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
实验方案
Product Specific Protocols | |
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WB protocol for CD33 antibody 67135-1-Ig | Download protocol |
IHC protocol for CD33 antibody 67135-1-Ig | Download protocol |
IF protocol for CD33 antibody 67135-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |