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CD33 Monoclonal antibody

CD33 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1B2E5

Cat no : 67135-1-Ig

Print datasheet

Synonyms

CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3

验证数据展示

  • WB analysis of THP-1 using 67135-1-Ig

    WB analysis of THP-1 using 67135-1-Ig

    THP-1 cells were subjected to SDS PAGE followed by western blot with 67135-1-Ig (CD33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human spleen using 67135-1-Ig

    IHC staining of human spleen using 67135-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human spleen using 67135-1-Ig

    IHC staining of human spleen using 67135-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67135-1-Ig (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 67135-1-Ig

    IF Staining of THP-1 using 67135-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD33 antibody (67135-1-Ig, Clone: 1B2E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

67135-1-Ig targets CD33 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen CD33 fusion protein Ag11402 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD33 molecule
Synonyms CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3
Calculated Molecular Weight 364 aa, 40 kDa
Observed Molecular Weight 67-70 kDa
GenBank Accession NumberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
RRIDAB_2882434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP20138
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

实验方案

Product Specific Protocols
WB protocol for CD33 antibody 67135-1-IgDownload protocol
IHC protocol for CD33 antibody 67135-1-IgDownload protocol
IF protocol for CD33 antibody 67135-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Front Med (Lausanne)

Prognostic significance of peripheral and tumor-infiltrating lymphocytes in newly diagnosed stage III/IV non-small-cell lung cancer

Authors - Fenge Li
View Article