CD33 Monoclonal antibody
CD33 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
1B3G4
验证数据展示
经过测试的应用
| Positive WB detected in | Mo7e cells, THP-1 cells, U-937 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67135-2-Ig targets CD33 in WB, ELISA applications and shows reactivity with Human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | Human |
| Immunogen | CD33 fusion protein Ag11402 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | CD33 molecule |
| Synonyms | CD33, CD33 molecule, FLJ00391, gp67, p67, SIGLEC 3, SIGLEC3 |
| Calculated Molecular Weight | 364 aa, 40 kDa |
| Observed Molecular Weight | 67-70 kDa |
| GenBank Accession Number | BC028152 |
| Gene Symbol | CD33 |
| Gene ID (NCBI) | 945 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20138 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD33 antibody 67135-2-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
