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CD33 Recombinant antibody

CD33 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241711D2

Cat no : 84341-3-RR

Print datasheet

Synonyms

SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67, CD33 molecule

验证数据展示

  • WB analysis using 84341-3-RR

    WB analysis using 84341-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84341-3-RR (CD33 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84341-3-RR

    IHC staining of human tonsillitis using 84341-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84341-3-RR

    IHC staining of human tonsillitis using 84341-3-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84341-3-RR (CD33 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inTHP-1 cells, K-562 cells, U-937 cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84341-3-RR targets CD33 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD33 molecule
Synonyms SIGLEC 3, Sialic acid-binding Ig-like lectin 3, p67, gp67, CD33 molecule
Calculated Molecular Weight 364 aa, 40 kDa
Observed Molecular Weight55-75 kDa
GenBank Accession NumberBC028152
Gene Symbol CD33
Gene ID (NCBI) 945
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP20138
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD33, also referred to as Siglec-3, is a 67-kDa glycosylated transmembrane protein of sialic acid-binding immunoglobulin-like lactic (siglec) family. CD33 is expressed on monocytes, myeloid progenitors, granulocytes, mast cells, some T cells. It may mediate cell-to-cell adhesion and act as a receptor that inhibits the proliferation of normal and leukemic myeloid cells.

实验方案

Product Specific Protocols
WB protocol for CD33 antibody 84341-3-RRDownload protocol
IHC protocol for CD33 antibody 84341-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols