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CD34 Polyclonal antibody

CD34 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat no : 31120-1-AP

Print datasheet

Synonyms

AU040960, Cd34, CD34 antigen

验证数据展示

  • IHC staining of mouse spleen using 31120-1-AP

    IHC staining of mouse spleen using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31120-1-AP

    IHC staining of mouse spleen using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse spleen tissue, mouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31120-1-AP targets CD34 in IHC, ELISA applications and shows reactivity with Mouse samples.

Tested Applications IHC, ELISA Application Description
Tested Reactivity Mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CD34 antigen
Synonyms AU040960, Cd34, CD34 antigen
Calculated Molecular Weight41 kDa
GenBank Accession NumberNM_133654
Gene Symbol Cd34
Gene ID (NCBI) 12490
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ64314
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
IHC protocol for CD34 antibody 31120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols