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验证数据展示

  • IHC staining of mouse spleen using 31120-1-AP

    IHC staining of mouse spleen using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse spleen using 31120-1-AP

    IHC staining of mouse spleen using 31120-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 31120-1-AP (CD34 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD34 Polyclonal antibody

CD34 Polyclonal Antibody for IHC, ELISA
Cat No. 31120-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Mouse

应用

IHC, ELISA

别名

AU040960, Cd34, CD34 antigen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse spleen tissue, mouse kidney tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31120-1-AP targets CD34 in IHC, ELISA applications and shows reactivity with Mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 Mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD34 antigen
别名 AU040960, Cd34, CD34 antigen
计算分子量41 kDa
GenBank蛋白编号NM_133654
基因名称 CD34
Gene ID (NCBI) 12490
RRIDAB_3669863
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ64314
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

实验方案

Product Specific Protocols
IHC protocol for CD34 antibody 31120-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols