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验证数据展示

  • WB analysis using 32211-1-AP

    WB analysis using 32211-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 32211-1-AP (CD34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 32211-1-AP

    IHC staining of human placenta using 32211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32211-1-AP (CD34 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IHC staining of human placenta using 32211-1-AP

    IHC staining of human placenta using 32211-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 32211-1-AP (CD34 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD34 antibody included
  • IF Staining of human placenta using 32211-1-AP

    IF Staining of human placenta using 32211-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CD34 antibody (32211-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD34 Polyclonal antibody

CD34 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 32211-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

CD34 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHL-60 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy CD34 Ready-To-Use IHC Kit. CD34 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

32211-1-AP targets CD34 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD34 molecule
别名 CD34 molecule
计算分子量41kDa
GenBank蛋白编号NM_001025109.2
基因名称 CD34
Gene ID (NCBI) 947
RRIDAB_3670223
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28906-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD34 is a 105- to 120-kDa glycophosphoprotein expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nerve tissue. CD34 is a commonly used marker for identifying human hematopoietic stem/progenitor cells and mediates cell adhesion and lymphocyte homing by binding L-selectin and E-selectin ligands. CD34 is also one of the best negative selection markers for characterizing and/or isolating human MSCs from bone marrow and other sources. Along with other positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification. The calculated molecular mass of human CD34 is 41 kDa, various forms with different molecular weights may be produced due to different glycosylation patterns and alternative splicing (PMID: 24375067; 15750786).

实验方案

Product Specific Protocols
WB protocol for CD34 antibody 32211-1-APDownload protocol
IHC protocol for CD34 antibody 32211-1-APDownload protocol
IF protocol for CD34 antibody 32211-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols