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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

    WB analysis of human heart using 66395-1-Ig (same clone as 66395-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 66395-1-Ig (CD36 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66395-1-Ig (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    IHC staining of human spleen using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 66395-1-Ig (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 66395-1-Ig (same clone as 66395-1-PBS)

    IF Staining of human liver cancer using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CD36 antibody (66395-1-Ig, Clone: 1A8C5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 66395-1-Ig (same clone as 66395-1-PBS)

    IF Staining of THP-1 using 66395-1-Ig (same clone as 66395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD36 antibody (66395-1-Ig, Clone: 1A8C5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66395-1-PBS in a different storage buffer formulation.

CD36 Monoclonal antibody, PBS Only

CD36 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 66395-1-PBS

产品说明书

CloneNo. 1A8C5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66395-1-PBS targets CD36 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 CD36 fusion protein Ag13541 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD36 molecule (thrombospondin receptor)
别名 GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb
计算分子量 472 aa, 53 kDa
观测分子量 88 kDa
GenBank蛋白编号BC008406
基因名称 CD36
Gene ID (NCBI) 948
RRIDAB_2811030
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP16671
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

  • Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

  • Polymorphisms in CD36 can increase susceptibility to malaria.

  • AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

  • Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.