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CD36 Recombinant antibody, PBS Only

CD36 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241262F5

Cat no : 84078-4-PBS

Print datasheet

Synonyms

GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb

验证数据展示

  • WB analysis using 84078-4-RR (same clone as 84078-4-PBS)

    WB analysis using 84078-4-RR (same clone as 84078-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84078-4-RR (CD36 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84078-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84078-4-RR (same clone as 84078-4-PBS)

    IHC staining of human placenta using 84078-4-RR (same clone as 84078-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84078-4-RR (CD36 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84078-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 84078-4-RR (same clone as 84078-4-PBS)

    IHC staining of human placenta using 84078-4-RR (same clone as 84078-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84078-4-RR (CD36 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84078-4-PBS in a different storage buffer formulation.

产品信息

The immunogen of 84078-4-PBS is CD36 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD36 molecule (thrombospondin receptor)
Synonyms GPIV, GPIIIB, GP4, GP3B, Glycoprotein IIIb
Calculated Molecular Weight53 kDa
Observed Molecular Weight75-85 kDa
GenBank Accession NumberNM_001001548.3
Gene Symbol CD36
Gene ID (NCBI) 948
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16671-1
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Function

CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.

Tissue specificity

CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.

Involvement in disease

  • Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.

  • Polymorphisms in CD36 can increase susceptibility to malaria.

  • AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.

  • Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.

Isoforms

Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).

Post-translational modifications

The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).

Cellular localization

CD36 is present on the cell surface.