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验证数据展示

  • WB analysis using 21044-1-AP

    WB analysis using 21044-1-AP

    Daudi cells were subjected to SDS PAGE followed by western blot with 21044-1-AP (CD37 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 21044-1-AP

    IHC staining of human tonsillitis using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 21044-1-AP

    IHC staining of human tonsillitis using 21044-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21044-1-AP (CD37 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD37 Polyclonal antibody

CD37 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21044-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

CD37 molecule, GP52 40, Leukocyte antigen CD37, Tetraspanin 26, Tspan 26

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21044-1-AP targets CD37 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 CD37 fusion protein Ag15384 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD37 molecule
别名 CD37 molecule, GP52 40, Leukocyte antigen CD37, Tetraspanin 26, Tspan 26
计算分子量 281 aa, 32 kDa
观测分子量40 kDa
GenBank蛋白编号BC110379
基因名称 CD37
Gene ID (NCBI) 951
RRIDAB_2878800
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11049
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

实验方案

Product Specific Protocols
WB protocol for CD37 antibody 21044-1-APDownload protocol
IHC protocol for CD37 antibody 21044-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

iScience

The clinical features, prognostic significance, and immune heterogeneity of CD37 in diffuse gliomas.

Authors - Xuejun Yan
View Article
mouseIHC

Sci Rep

89Zr-PET imaging to predict tumor uptake of 177Lu-NNV003 anti-CD37 radioimmunotherapy in mouse models of B cell lymphoma.

Authors - Danique Giesen
View Article