验证数据展示
经过测试的应用
| Positive WB detected in | rat spleen tissue, HeLa cells, human spleen tissue |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 2 publications below |
| IF | See 1 publications below |
产品信息
21044-1-AP targets CD37 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | IHC, IF |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CD37 fusion protein Ag15384 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD37 molecule |
| Synonyms | TSPAN26, Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40 |
| Calculated Molecular Weight | 281 aa, 32 kDa |
| Observed Molecular Weight | 40-52 kDa |
| GenBank Accession Number | BC110379 |
| Gene Symbol | CD37 |
| Gene ID (NCBI) | 951 |
| RRID | AB_2878800 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11049 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD37 antibody 21044-1-AP | Download protocol |
| IHC protocol for CD37 antibody 21044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience The clinical features, prognostic significance, and immune heterogeneity of CD37 in diffuse gliomas. | ||
Sci Rep 89Zr-PET imaging to predict tumor uptake of 177Lu-NNV003 anti-CD37 radioimmunotherapy in mouse models of B cell lymphoma. |
