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验证数据展示

  • WB analysis using 84509-2-RR

    WB analysis using 84509-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84509-2-RR (CD37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84509-2-RR

    IHC staining of human tonsillitis using 84509-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84509-2-RR

    IHC staining of human tonsillitis using 84509-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Daudi using 84509-2-RR

    IF Staining of Daudi using 84509-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD37 antibody (84509-2-RR, Clone: 242011D6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD37 Recombinant antibody

CD37 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84509-2-RR

Print datasheet

CloneNo. 242011D6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inDaudi cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84509-2-RR targets CD37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD37 molecule
Synonyms Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule
Calculated Molecular Weight32kDa
Observed Molecular Weight40-52 kDa
GenBank Accession NumberNM_001774.3
Gene Symbol CD37
Gene ID (NCBI) 951
RRIDAB_3672018
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP11049-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

实验方案

Product Specific Protocols
WB protocol for CD37 antibody 84509-2-RRDownload protocol
IHC protocol for CD37 antibody 84509-2-RRDownload protocol
IF protocol for CD37 antibody 84509-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols