• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84509-2-RR

    WB analysis using 84509-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84509-2-RR (CD37 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84509-2-RR

    IHC staining of human tonsillitis using 84509-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 84509-2-RR

    IHC staining of human tonsillitis using 84509-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84509-2-RR (CD37 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Daudi using 84509-2-RR

    IF Staining of Daudi using 84509-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using CD37 antibody (84509-2-RR, Clone: 242011D6 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CD37 Recombinant antibody

CD37 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 84509-2-RR

产品说明书

CloneNo. 242011D6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inDaudi cells, Raji cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inDaudi cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84509-2-RR targets CD37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 FusionProtein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD37 molecule
别名 Tspan 26, Tetraspanin 26, Leukocyte antigen CD37, GP52 40, CD37 molecule
计算分子量32kDa
观测分子量40-52 kDa
GenBank蛋白编号NM_001774.3
基因名称 CD37
Gene ID (NCBI) 951
RRIDAB_3672018
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP11049-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477).

实验方案

Product Specific Protocols
WB protocol for CD37 antibody 84509-2-RRDownload protocol
IHC protocol for CD37 antibody 84509-2-RRDownload protocol
IF protocol for CD37 antibody 84509-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols