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CD38 Recombinant antibody

CD38 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

241246B1

Cat no : 84070-2-RR

Print datasheet

Synonyms

ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase

验证数据展示

  • WB analysis using 84070-2-RR

    WB analysis using 84070-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84070-2-RR (CD38 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 84070-2-RR

    IHC staining of human tonsillitis using 84070-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-2-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD38 antibody included
  • IHC staining of human tonsillitis using 84070-2-RR

    IHC staining of human tonsillitis using 84070-2-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84070-2-RR (CD38 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CD38 antibody included
  • IF Staining of Raji using 84070-2-RR

    IF Staining of Raji using 84070-2-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD38 antibody (84070-2-RR, Clone: 241246B1 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84070-2-RR

    Affinity and Kinetic Characterization of 84070-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84070-2-RR against Human CD38 were performed. The affinity constant is 0.999 nM.

经过测试的应用

Positive WB detected inRaji cells, A549 cells, MOLT-4 cells, Ramos cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells
Planning an IHC experiment? We recommend our IHCeasy CD38 Ready-To-Use IHC Kit. CD38 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84070-2-RR is CD38 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD38 molecule
Synonyms ADPRC 1, ADP ribosyl cyclase 1, 2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase, 2'-phospho-ADP-ribosyl cyclase
Calculated Molecular Weight 300 aa, 34 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC007964
Gene Symbol CD38
Gene ID (NCBI) 952
ENSEMBL Gene IDENSG00000004468
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP28907
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD38, also known as ADP-ribosyl cyclase 1, is a type II transmembrane glycoprotein with a short N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites (PMID: 2319135). The extracellular domain of CD38 has bifunctional enzyme activities that catalyze synthesis of cyclic ADP ribose from nicotinamide adenine dinucleotide (NAD) and hydrolysis of cyclic ADP ribose to adenosine diphosphoribose (PMID: 10636863). CD38 is expressed on a variety of hematopoietic and non-hematopoietic cells and is involved in diverse processes such as generation of calcium-mobilizing metabolites, cell activation, and chemotaxis (PMID: 25938500).

实验方案

Product Specific Protocols
WB protocol for CD38 antibody 84070-2-RRDownload protocol
IHC protocol for CD38 antibody 84070-2-RRDownload protocol
IF protocol for CD38 antibody 84070-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols