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验证数据展示

  • WB analysis using 84214-4-RR (same clone as 84214-4-PBS)

    WB analysis using 84214-4-RR (same clone as 84214-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84214-4-RR (CD3 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84214-4-RR (CD3 Delta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84214-4-RR (CD3 Delta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    IF Staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD3 Delta antibody (84214-4-RR, Clone: 241523H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01149-2

    Cytometric bead array standard curve of MP01149-2

    Cytometric bead array standard curve of MP01149-2, CD3D Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84214-1-PBS. Detection antibody: 84214-4-PBS. Standard: Eg2288. Range: 0.625-80 ng/mL.

CD3 Delta Recombinant antibody, PBS Only (Detector)

CD3 Delta Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, Cytometric bead array, Indirect ELISA
Cat No. 84214-4-PBS

产品说明书

CloneNo. 241523H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, Cytometric bead array, Indirect ELISA

别名

CD3D, 241523H2, T-cell receptor T3 delta chain, T3D, T cell receptor T3 delta chain

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84214-4-PBS targets CD3 Delta as part of a matched antibody pair:

MP01149-2: 84214-1-PBS capture and 84214-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF-P, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD3 Delta fusion protein Eg2288 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD3d molecule, delta (CD3-TCR complex)
别名 CD3D, 241523H2, T-cell receptor T3 delta chain, T3D, T cell receptor T3 delta chain
计算分子量 171 aa, 19 kDa
观测分子量20-25 kDa
GenBank蛋白编号BC070321
基因名称 CD3 Delta
Gene ID (NCBI) 915
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04234
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. CD3 delta/CD3D is a single-pass type I membrane protein which consists of an extracellular domain of 84 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. Defects in CD3 delta are a cause of severe combined immunodeficiency.