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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 31162-1-AP (same clone as 31162-1-PBS)

    WB analysis using 31162-1-AP (same clone as 31162-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31162-1-AP (CD3EAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31162-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31162-1-AP (same clone as 31162-1-PBS)

    IF Staining of A431 using 31162-1-AP (same clone as 31162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (31162-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31162-1-PBS in a different storage buffer formulation.

CD3EAP Polyclonal antibody, PBS Only

CD3EAP Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31162-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

31162-1-PBS targets CD3EAP in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD3EAP fusion protein Ag35074 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CD3e molecule, epsilon associated protein
别名
计算分子量 510 aa, 55 kDa
观测分子量80-90 kDa
GenBank蛋白编号BC038992
基因名称 CD3EAP
Gene ID (NCBI) 10849
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO15446
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.