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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using 65603-1-MR

    FC experiment of human PBMCs using 65603-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 65603-1-MR

    FC experiment of human PBMCs using 65603-1-MR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Mouse IgG2a Recombinant Antibody (65603-1-MR, Clone: OKT4) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated.

Anti-Human CD4 (OKT4) Mouse IgG2a Recombinant Antibody

CD4 Uni-rAbTM Recombinant Antibody for FC
Cat No. 65603-1-MR

Print datasheet

CloneNo. OKT4

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

65603-1-MR targets CD4 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen N/A 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Recombinant
Type Antibody
Full Name CD4 molecule
Synonyms T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3670350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for CD4 antibody 65603-1-MRDownload protocol
Standard Protocols
Click here to view our Standard Protocols