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验证数据展示

  • WB analysis of U-937 using 83513-6-RR

    WB analysis of U-937 using 83513-6-RR

    U-937 cells were subjected to SDS PAGE followed by western blot with 83513-6-RR (CD4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83513-6-RR

    Affinity and Kinetic Characterization of 83513-6-RR

    Biolayer interferometry (BLl) kinetic assays of 83513-6-RR against Human CD4 were performed. The affinity constant is 0.391 nM.

CD4 Recombinant antibody

CD4 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83513-6-RR

Print datasheet

CloneNo. 240427B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427B10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inU-937 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83513-6-RR targets CD4 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD4 molecule
Synonyms T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427B10
Calculated Molecular Weight 55 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3671137
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
WB protocol for CD4 antibody 83513-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols