验证数据展示
经过测试的应用
Positive WB detected in | U-937 cells, THP-1 cells |
Positive IF/ICC detected in | THP-1 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IF | See 1 publications below |
产品信息
83513-7-RR targets CD4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
文献引用应用 | IF |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | Fusion Protein 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | CD4 molecule |
别名 | T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12 |
计算分子量 | 55 kDa |
观测分子量 | 55 kDa |
GenBank蛋白编号 | BC025782 |
基因名称 | CD4 |
Gene ID (NCBI) | 920 |
ENSEMBL Gene ID | ENSG00000010610 |
RRID | AB_3671138 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | P01730 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).
实验方案
Product Specific Protocols | |
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WB protocol for CD4 antibody 83513-7-RR | Download protocol |
IF protocol for CD4 antibody 83513-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |