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CD4 Recombinant antibody

CD4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

240427E12

Cat no : 83513-7-RR

Print datasheet

Synonyms

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12

验证数据展示

  • WB analysis using 83513-7-RR

    WB analysis using 83513-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83513-7-RR (CD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of THP-1 using 83513-7-RR

    IF Staining of THP-1 using 83513-7-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CD4 antibody (83513-7-RR, Clone: 240427E12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • Affinity and Kinetic Characterization of 83513-7-RR

    Affinity and Kinetic Characterization of 83513-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83513-7-RR against Human CD4 were performed. The affinity constant is 0.426 nM.

经过测试的应用

Positive WB detected inU-937 cells, THP-1 cells
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

83513-7-RR targets CD4 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD4 molecule
Synonyms T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4mut, CD4 molecule, 240427E12
Calculated Molecular Weight 55 kDa
Observed Molecular Weight55 kDa
GenBank Accession NumberBC025782
Gene Symbol CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3671138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP01730
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
WB protocol for CD4 antibody 83513-7-RRDownload protocol
IF protocol for CD4 antibody 83513-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

J Transl Med

Tumor microenvironment remodeling after neoadjuvant chemoradiotherapy in local advanced rectal cancer revealed by single-cell RNA sequencing

Authors - Wenzhao Su
View Article