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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of human PBMCs using Biotin-65505

    FC experiment of human PBMCs using Biotin-65505

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD4 Rabbit IgG Recombinant Antibody (Biotin-65505, Clone: OKT4) and CoraLite®488-conjugated streptavidin, or unstained. Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using Biotin-65505

    FC experiment of human PBMCs using Biotin-65505

    1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human CD4 Rabbit IgG Recombinant Antibody (Biotin-65505, Clone: OKT4) and CoraLite®488-conjugated streptavidin (red), or unstained (blue). Cells were not fixed. Lymphocytes were gated.

Biotin Anti-Human CD4 (OKT4) Rabbit IgG Recombinant Antibody

CD4 Recombinant Antibody for FC
Cat No. Biotin-65505

产品说明书

CloneNo. OKT4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-65505 targets CD4 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human peripheral T cells 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD4 molecule
别名 T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3672371
偶联类型 Biotin
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for Biotin CD4 antibody Biotin-65505Download protocol
Standard Protocols
Click here to view our Standard Protocols