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CoraLite® Plus 488 Anti-Mouse CD4 (GK1.5)

CD4 Monoclonal Antibody for IF/ICC, FC

Host / Isotype

Rat / IgG2b, kappa

Reactivity

mouse

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

GK1.5

Cat no : CL488-65104

Print datasheet

Synonyms

Ly 4, L3T4, GK1.5, CD4 antigen

验证数据展示

  • FC experiment of mouse splenocytes using CL488-65104

    FC experiment of mouse splenocytes using CL488-65104

    1X10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 488 Anti-Mouse CD4 (CL488-65104, Clone:GK1.5) or 0.25 ug CoraLite® Plus 488 Rat IgG2b Isotype Control (LTF-2) (CL488-65211, Clone: LTF-2), and 0.25 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.

  • IF Staining of mouse splenocytes using CL488-65104

    IF Staining of mouse splenocytes using CL488-65104

    Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated Anti-Mouse CD4 (CL488-65104, Clone: GK1.5) at dilution of 1:500.

经过测试的应用

Positive IF/ICC detected inmouse splenocytes
Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65104-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-65104 targets CD4 in IF/ICC, FC applications and shows reactivity with mouse samples.

Tested Applications IF/ICC, FC Application Description
Tested Reactivity mouse
Immunogen Mouse CTL clone V4 种属同源性预测
Host / Isotype Rat / IgG2b, kappa
Class Monoclonal
Type Antibody
Full Name CD4 antigen
Synonyms Ly 4, L3T4, GK1.5, CD4 antigen
GenBank Accession NumberBC039137
Gene Symbol Cd4
Gene ID (NCBI) 12504
RRIDAB_2883178
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDP06332
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD4 antibody CL488-65104Download protocol
Standard Protocols
Click here to view our Standard Protocols