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验证数据展示

  • FC experiment of human PBMCs using FITC-65505

    FC experiment of human PBMCs using FITC-65505

    1x10^6 human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human CD4 Rabbit Recombinant Antibody (FITC-65505, Clone:OKT4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using FITC-65505

    FC experiment of human PBMCs using FITC-65505

    1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD4 Rabbit Recombinant Antibody (FITC-65505, Clone:OKT4) or FITC-conjugated Rabbit IgG control PolyAb (FITC-30000). Cells were not fixed. Lymphocytes were gated.

FITC Plus Anti-Human CD4 (OKT4) Rabbit IgG Recombinant Antibody

CD4 Recombinant Antibody for FC
Cat No. FITC-65505

产品说明书

CloneNo. OKT4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  {{ptg:cur_Conjugation}}
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

应用推荐稀释比
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-65505 targets CD4 in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Human peripheral T cells 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD4 molecule
别名 T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, OKT4, CD4mut, CD4 molecule
计算分子量 55 kDa
GenBank蛋白编号BC025782
基因名称 CD4
Gene ID (NCBI) 920
ENSEMBL Gene IDENSG00000010610
RRIDAB_3673847
偶联类型 FITC Plus Fluorescent Dye
最大激发/发射波长495 nm / 524 nm
形式 Liquid
纯化方式Affinity purification
储存缓冲液 PBS with 0.09% sodium azide and 0.5% BSA , pH 7.3
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

实验方案

Product Specific Protocols
FC protocol for FITC Plus CD4 antibody FITC-65505Download protocol
Standard Protocols
Click here to view our Standard Protocols