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验证数据展示

  • WB analysis of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

    WB analysis of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IF Staining of Ramos using 66965-1-Ig (same clone as 66965-1-PBS)

    IF Staining of Ramos using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Ramos cells using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

CD40 Monoclonal antibody, PBS Only

CD40 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 66965-1-PBS

产品说明书

CloneNo. 2A8G5

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66965-1-PBS targets CD40 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human
免疫原 CD40 fusion protein Ag28040 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CD40 molecule, TNF receptor superfamily member 5
别名 p50, CD40L receptor, Bp50, B-cell surface antigen CD40, B cell surface antigen CD40
计算分子量 277 aa, 31 kDa
观测分子量 43 kDa
GenBank蛋白编号BC012419
基因名称 CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
RRIDAB_2882288
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25942
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.