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CD40 Monoclonal antibody, PBS Only

CD40 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2A8G5

Cat No : 66965-1-PBS

Print datasheet

Synonyms

B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5

验证数据展示

  • WB analysis of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

    WB analysis of Raji using 66965-1-Ig (same clone as 66965-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66965-1-Ig (CD40 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IHC staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66965-1-Ig (CD40 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    IF Staining of human tonsillitis using 66965-1-Ig (same clone as 66965-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD40 antibody (66965-1-Ig, Clone: 2A8G5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66965-1-PBS in a different storage buffer formulation.

产品信息

66965-1-PBS targets CD40 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen CD40 fusion protein Ag28040 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CD40 molecule, TNF receptor superfamily member 5
Synonyms B cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDW40, p50, TNFRSF5
Calculated Molecular Weight 277 aa, 31 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC012419
Gene Symbol CD40
Gene ID (NCBI) 958
ENSEMBL Gene IDENSG00000101017
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25942
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.