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Species Reactivity
0
Conjugate
0
Conjugate
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0
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验证数据展示

  • WB analysis using 83972-5-RR (same clone as 83972-5-PBS)

    WB analysis using 83972-5-RR (same clone as 83972-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83972-5-RR (CD40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83972-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83972-5-RR

    Affinity and Kinetic Characterization of 83972-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83972-5-RR against Mouse CD40 were performed. The affinity constant is 4.50 nM.

Mouse CD40 Recombinant antibody, PBS Only

CD40 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 83972-5-PBS

产品说明书

CloneNo. 240874B2

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, Indirect ELISA

别名

Tnfrsf5, CD40 antigen, Bp50, 240874B2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83972-5-PBS targets CD40 in WB, Indirect ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 CD40 antigen
别名 Tnfrsf5, CD40 antigen, Bp50, 240874B2
计算分子量32 kDa
观测分子量40-45 kDa
GenBank蛋白编号NM_011611.2
基因名称 Cd40
Gene ID (NCBI) 21939
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP27512-1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.