验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, human peripheral blood platelets |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 3 publications below |
| IF | See 1 publications below |
产品信息
66502-1-Ig targets CD40L/CD154 in WB, IHC, IF, ELISA applications and shows reactivity with Human, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | IHC, IF |
| 经测试反应性 | Human, rat |
| 文献引用反应性 | human |
| 免疫原 | CD40L/CD154 fusion protein Ag24941 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | CD40 ligand |
| 别名 | CD154, CD40 L, CD40 ligand, CD40L, CD40L/CD154, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP |
| 计算分子量 | 261 aa, 29 kDa |
| 观测分子量 | 30 kDa |
| GenBank蛋白编号 | BC071754 |
| 基因名称 | CD40 Ligand |
| Gene ID (NCBI) | 959 |
| RRID | AB_2881866 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P29965 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD40L/CD154 antibody 66502-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Thyroid A MULTICENTER, SINGLE-BLIND, CASE-CONTROL, IMMUNOHISTOCHEMICAL STUDY OF ORBITAL TISSUE IN THYROID EYE DISEASE | ||
Pathol Res Pract The potential role of follicular helper T cells and helper T cells type 1 in Warthin tumour. | ||
Pathol Res Pract The potential role of follicular helper T cells in idiopathic multicentric Castleman disease with and without TAFRO syndrome. | ||
Br J Cancer Integrated proteomics and scRNA-seq analyses of ovarian cancer reveal molecular subtype-associated cell landscapes and immunotherapy targets |
