CD44 Polyclonal antibody
CD44 Polyclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse, Rat and More (1)
Applications
WB, IF/ICC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | J774A.1 cells, RAW 264.7 cells, NR8383 cells |
| Positive IF/ICC detected in | RAW 264.7 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
30854-1-AP targets CD44 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Mouse, Rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Cited Applications | IHC |
| Tested Reactivity | Mouse, Rat |
| Cited Reactivity | rat |
| Immunogen | CD44 fusion protein Ag33515 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CD44 antigen |
| Synonyms | CD44, CD44 antigen, CDw44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, HERMES, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, Ly 24, MC56, MDU2, MDU3, MIC4, MUTCH I, Pgp 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I |
| Calculated Molecular Weight | 86kd |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | NM_009851 |
| Gene Symbol | Cd44 |
| Gene ID (NCBI) | 12505 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P15379 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD44 antibody 30854-1-AP | Download protocol |
| IF protocol for CD44 antibody 30854-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |