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  • Featured Product
  • KD/KO Validated

CD44 Monoclonal antibody, PBS Only

CD44 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

6F4H2

Cat no : 60224-1-PBS

Print datasheet

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

验证数据展示

  • WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

    WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

    IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

    IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using 60224-1-Ig (CD44 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD44 antibody included
  • WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

    WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

    WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

产品信息

60224-1-PBS targets CD44 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
Tested Reactivity human, pig
Immunogen CD44 fusion protein Ag7633 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name CD44 molecule (Indian blood group)
Synonyms ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen
Calculated Molecular Weight 742 aa, 82 kDa
Observed Molecular Weight 80-95 kDa
GenBank Accession NumberBC004372
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
RRIDAB_11042767
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16070
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.