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CD44 Recombinant antibody

CD44 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241648D1

Cat no : 84369-5-RR

Print datasheet

Synonyms

Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen

验证数据展示

  • WB analysis of rat spleen using 84369-5-RR

    WB analysis of rat spleen using 84369-5-RR

    rat spleen tissue was subjected to SDS PAGE followed by western blot with 84369-5-RR (CD44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat spleen using 84369-5-RR

    IHC staining of rat spleen using 84369-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat spleen using 84369-5-RR

    IHC staining of rat spleen using 84369-5-RR

    Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84369-5-RR

    Affinity and Kinetic Characterization of 84369-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84369-5-RR against Rat CD44 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inrat spleen tissue
Positive IHC detected inrat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84369-5-RR targets CD44 in WB, IHC, ELISA applications and shows reactivity with rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity rat
Immunogen FusionProtein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name Cd44 molecule
Synonyms Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen
Calculated Molecular Weight40 kDa
Observed Molecular Weight80 kDa
GenBank Accession NumberXM_006234631.3
Gene Symbol Cd44
Gene ID (NCBI) 25406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO70509
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 84369-5-RRDownload protocol
IHC protocol for CD44 antibody 84369-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols