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CD44 Recombinant antibody

CD44 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

242345G11

Cat no : 84854-5-RR

Print datasheet

Synonyms

ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen

验证数据展示

  • WB analysis using 84854-5-RR

    WB analysis using 84854-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84854-5-RR (CD44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84854-5-RR

    IF Staining of A431 using 84854-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (84854-5-RR, Clone: 242345G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84854-5-RR

    Affinity and Kinetic Characterization of 84854-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84854-5-RR against Human CD44 were performed. The affinity constant is 1.01 nM.

经过测试的应用

Positive WB detected inHeLa cells, MDA-MB-231 cells, A549 cells, PC-3 cells, PANC-1 cells
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CD44 Ready-To-Use IHC Kit. CD44 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84854-5-RR targets CD44 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name CD44 molecule (Indian blood group)
Synonyms ECMR-III, ECMR III, CSPG8, CDW44, CD44 antigen
Calculated Molecular Weight82 kDa
Observed Molecular Weight80-95 kDa
GenBank Accession NumberNM_000610.3
Gene Symbol CD44
Gene ID (NCBI) 960
ENSEMBL Gene IDENSG00000026508
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP16070-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

实验方案

Product Specific Protocols
WB protocol for CD44 antibody 84854-5-RRDownload protocol
IF protocol for CD44 antibody 84854-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols