CD45.1 Recombinant antibody

CD45.1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 84325-2-RR
CloneNo. 240921A2

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF-P, ELISA

Ly5a, Cd45, Ptprc, L-CA, LCA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inSJL mouse thymus tissue, SJL mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse spleen tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84325-2-RR targets CD45.1 in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms Ly5a, Cd45, Ptprc, L-CA, LCA
Observed Molecular Weight200 kDa
Gene Symbol CD45
Gene ID (NCBI) 19264
RRIDAB_3671867
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). Allelic variants of mouse CD45, CD45.1 (Ly5.1) and CD45.2 (Ly5.1), have been established as a marker system to track haematopoietic cells following congenic mouse bone marrow transplants (PMID: 28615666). CD45.2 is the common form and is expressed by most of the established strains, while CD45.1 is found in only a few like the SJL mouse strain (PMID: 3489673).

实验方案

Product Specific Protocols
WB protocol for CD45.1 antibody 84325-2-RRDownload protocol
IHC protocol for CD45.1 antibody 84325-2-RRDownload protocol
IF protocol for CD45.1 antibody 84325-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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