CD45 Polyclonal antibody
CD45 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, Raji cells, Ramos cells |
Positive IHC detected in | human tonsillitis tissue, human liver cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
29914-1-AP targets CD45 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | CD45 fusion protein Ag31286 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | protein tyrosine phosphatase, receptor type, C |
Synonyms | LCA, L CA, GP180, EC:3.1.3.48, CD45R |
Calculated Molecular Weight | 1304 aa, 147 kDa |
Observed Molecular Weight | 180-250 kDa |
GenBank Accession Number | BC014239 |
Gene Symbol | CD45 |
Gene ID (NCBI) | 5788 |
ENSEMBL Gene ID | ENSG00000081237 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P08575 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD45 antibody 29914-1-AP | Download protocol |
IHC protocol for CD45 antibody 29914-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |