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CD45 Polyclonal antibody

CD45 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 31243-1-AP

Print datasheet

Synonyms

B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200

验证数据展示

  • WB analysis using 31243-1-AP

    WB analysis using 31243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31243-1-AP (CD45/PTPRC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inEL-4 cells, J774A.1 cells, RAW 264.7 cells, mouse spleen tissue, mouse thymus tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31243-1-AP targets CD45 in WB, ELISA applications and shows reactivity with Mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein tyrosine phosphatase, receptor type, C
Synonyms B220, Cd45, CD45R, loc, Ly 5, Lyt 4, Ptprc, T200
Calculated Molecular Weight145 kDa
Observed Molecular Weight180 kDa
GenBank Accession NumberNM_001111316.2
Gene Symbol Ptprc
Gene ID (NCBI) 19264
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06800-4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
WB protocol for CD45 antibody 31243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols