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Species Reactivity
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Species Reactivity
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 31243-1-AP

    WB analysis using 31243-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31243-1-AP (CD45/PTPRC antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of RAW 264.7 using 31243-1-AP

    IF Staining of RAW 264.7 using 31243-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CD45 antibody (31243-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CD45 Polyclonal antibody

CD45 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 31243-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

WB, IF/ICC, ELISA

别名

Ptprc, Leukocyte common antigen, LCA, B220

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inEL-4 cells, J774A.1 cells, RAW 264.7 cells, mouse spleen tissue, mouse thymus tissue
Positive IF/ICC detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31243-1-AP targets CD45 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 Ptprc, Leukocyte common antigen, LCA, B220
计算分子量145 kDa
观测分子量180 kDa
GenBank蛋白编号NM_001111316.2
基因名称 CD45
Gene ID (NCBI) 19264
RRIDAB_3669915
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP06800-4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

实验方案

Product Specific Protocols
WB protocol for CD45 antibody 31243-1-APDownload protocol
IF protocol for CD45 antibody 31243-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols