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Species Reactivity
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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 80297-1-RR (same clone as 80297-1-PBS)

    WB analysis using 80297-1-RR (same clone as 80297-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80297-1-RR (CD45 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    IHC staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • IF Staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    IF Staining of mouse colon using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • IF Staining of Jurkat using 80297-1-RR (same clone as 80297-1-PBS)

    IF Staining of Jurkat using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

    IF Staining of mouse spleen using 80297-1-RR (same clone as 80297-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD45 antibody (80297-1-RR, Clone: 6O19 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 80297-1-RR (same clone as 80297-1-PBS)

    IHC staining of human tonsillitis using 80297-1-RR (same clone as 80297-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80297-1-RR (CD45 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80297-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD45 antibody included
  • Affinity and Kinetic Characterization of 80297-1-RR

    Affinity and Kinetic Characterization of 80297-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80297-1-RR against Human CD45 were performed. The affinity constant is 1.61 nM.

CD45 Recombinant antibody, PBS Only

CD45 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA
Cat No. 80297-1-PBS

产品说明书

CloneNo. 6O19

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA

别名

LCA, L CA, GP180, EC:3.1.3.48, B220

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CD45 Ready-To-Use IHC Kit. CD45 primary antibody included.

产品信息

80297-1-PBS targets CD45 in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA Application Description
经测试反应性 human, mouse
免疫原 CD45 fusion protein Ag13712 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, C
别名 LCA, L CA, GP180, EC:3.1.3.48, B220
计算分子量 1304 aa, 147 kDa
观测分子量220-240 kDa
GenBank蛋白编号BC014239
基因名称 CD45
Gene ID (NCBI) 5788
ENSEMBL Gene IDENSG00000081237
RRIDAB_2918884
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP08575
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.